The United States Department of Transportation (D.O.T.) is a department of the U.S. Federal Government, which oversees all issues regarding transportation within the United States of America and U.S. Territories. Its influence around the world is great and widely respected, but its jurisdiction and power of enforcement is limited to the USA and its territories. As regards this paper, we will discuss the D.O.T. and its involvement surrounding sample cylinders for the hydrocarbon industry and the rules regarding the movement of these cylinders from point to point in the United States